Technical improvements in measurement of L -aspartate:2-oxoglutarate aminotransferase activity in serum by diazonium salt coupling.
نویسندگان
چکیده
3. Jacobs, W. L. W., Gamrna-glutamyl transpeptidase in disease of the liver, cardiovascularsystem and diabetesmellitus.Clin. Chim. Acta 38,419(1972). 4. Karmen, A., A note on the spectrophotometric assay of glutamic oxalacetic transaminase in human blood serum. J. Clin. Invest. 34, 131 (1955). 5. Wroblewski, F., and La Due, J. S., Serum glutamic pyruvic transaminase in cardiac and hepatic disease. Proc. Soc. Exp. Biol. 91, 569(1956). 6. Hochella, N. J., and Weinhouse, S., Automated lactic acid determination in serum and tissue extracts. Anal. Biochem. 13, 322 (1965). 7. Marsh, W. H., Fingerhut, B., and Kirsch, E., Adaptation of an alkaline phosphatase method for automatic colorimetric analysis.
منابع مشابه
Assay of aspartate aminotransferase activity: effects of serum and serum proteins on oxalacetate decarboxylation and dialysis.
Effects of serum and protein on assays measuring aspartate aminotransferase (L-aspartate: 2-oxoglutarate aminotransferase, EC 2.6.1.1) were examined. Increasing amounts of normal human serum or human serum albumin in samples with fixed amounts of purified enzyme decrease absorbance values and thus distort the results obtained in an assay in which diazonium-salt chromogenic reagent is used. Seru...
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A method for measuring serum glutamic oxalacetic transaminase activity is described, in which substrate concentrations are more nearly optimal than in previous colonmetric methods. By coupling the diazonium salt at a pH of 4.2, interference from a-oxoglutarate is negligible. Oxalacetate is produced at a linear rate to 180 l.U. Results correlate well with those obtained by a reference spectropho...
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Serum, 2 ml, was assayed colorimetrically with the “SMA 12/60 AutoAnalyzer” (Technicon Instruments Corp., Tarrytown, N.Y. 10591). Colorimetric determination (at 45#{176}C) of AST with the SMA 12/60 is based on the coupling reaction of a diazonium salt of N-butyl4-methoxymetanilamide with oxaloacetic acid produced when AST acts on ketoglutarate. AST activity of the same serum specimen was determ...
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Oxamate, a potent inhibitor of lactate dehydrogenase, is shown also to inhibit aspartate aminotransferase activity, both in human serum and in purified isoenzymes of human origin. The inhibition was competitive with respect to 2-oxoglutarate for both isoenzymes. The apparent Ki was 29 mmol/L for the cytoplasmic enzyme and 17 mmol/L for the mitochondrial enzyme. Noncompetitive inhibition was fou...
متن کاملAzide as a preservative in assays of aspartate aminotransferase activity.
Azide at an effective antimicrobial concentration, 7.7 mmol/liter, inhibits neither the cytoplasmic or the mitochondrial isoenzymes of human aspartate aminotransferase (EC 2.6. 1. 1) or porcine malate dehydrogenase (EC 1.1.1.37). It markedly prolongs the usefulness of substrates in assays for estimating aspartate aminotransferase activity (aspartate substrate without azide deteriorates in less ...
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عنوان ژورنال:
- Clinical chemistry
دوره 19 7 شماره
صفحات -
تاریخ انتشار 1973